Further Information
Internal field trials were performed by ourselves to verify that the Dosatron provided a suitable means of vaccine application. The trial consisted of performing a vaccination using the following methods;
Header Tank
Gumboro vaccine was reconstituted and mixed into a header tank with the required amount of water stabiliser. The site was fitted with 12 water lines which were primed so the dye was present at the end of all lines. A sample was taken from each line for Virus Recovery at Scitech Laboratories using the TC1D50 Tissue Culture 50% endpoint method calculated by the Method of Reed and Muench.
Dosatron Method
Gumboro vaccine was reconstituted into a stock solution with the required amount of water stabiliser. All water lines were primed with vaccine solution and a sample was taken from each water line for analysis.
Results
Header Tank
The header tank vaccine virus recovery work showed incosistend application results with an uneven spread of vaccine down all lines. In ten of the twelve water lines an average of 100 virus particles per millilitre were present per millimetre while in a single line a reading of 10³ (approx. 1000 virus particles) was recorded. A low reading of sub 10¹ which a trace reading was present in line four.
Dosatron
The results of the field showed an even spread of vaccine virus down all lines with an average of approximately 10³ (1000 virus particles per millilitre).
Conclusion
In our opinion the Dosatron units offer a reliable and highly accurate method of applying live vaccine via the water lines.
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